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mir-140 is downstream of Sox9 transcription factors. Riboprobe detection of pri-miR-140 in histological sections of wild-type 3 dpf embryos (A), a sox9a mutant (B), a sox9b mutant (C), and a sox9a;sox9b double mutant (D). The lack of expression of mir-140 in sox9a and sox9b mutants shows that Sox9 acts upstream of mir-140. ey, eye; pa, pharyngeal arches; WT, wild type.
