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Determination of JH-II-126:PhoQcat dissociation constant. Titration of JH-II-126 into a TNP-ATP:PhoQcat complex solution displaces TNP-ATP, leading to a decrease in relative fluorescence. The Kd of the JH-II-126:PhoQcat interaction determined from this competition assay is 391 ± 61 μM. Error bars represent standard deviation of n = 3 values.
