Skip to main content
. 2011 Jan 6;17(4):435–449. doi: 10.1089/ten.tec.2009.0510

FIG. 8.

FIG. 8.

Histological sections of naked islets and islets encapsulated in alginate and PFC alginate. (A) Normal alginate microcapsule after 2 days of culture in 20% oxygen. Asterisk (*) identifies central necrosis in the larger islet indicated by light staining at the islet center. (B, E) PFC alginate microcapsule on the day of encapsulation. Dead cells are observed at the islet exterior (arrows). (C) 70% (w/v) PFC alginate microcapsule after 2 days of culture in 20% O2. There are dead cells in a small cap of tissue at the capsule edge (arrow). (D) Normal alginate microcapsule after 2 days of culture in 3% O2. Asterisk (*) identifies central necrosis. (F) PFC alginate microcapsule after 2 days of culture in 3% oxygen. The tissue at the center of the islet appears healthy, and there are no signs of central necrosis. Dead cells surrounded the islet exterior (arrows). (G–I) Naked islets after 2 days of culture: (G) Naked islet in islet culture media; tissue appears healthy. (H) Naked islets cultured in islet culture media on top of pure perfluorodecalin; clumping occurred, but tissue appears healthy. (I) Naked islets cultured in islet culture media containing Intralipid®. Dead cells are observed at islet periphery (arrows).