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The kinetics of EPO effect on ROS generation by RBC and platelets. A diluted blood sample obtained from a thalassemia patient was stained with DCF, washed, and then incubated at room temperature with Epo (1 U/ml). Fluorescence was measured by flow cytometry at the indicated time points. The time-related changes in the mean fluorescent intensity (MFI) of each population are indicated.
