Figure 3.

Effect of Epo on the oxidative stress of RBC and platelets from thalassemic patients. Diluted blood samples were untreated with or without 1 U/ml Epo for 3 hrs at 37°C, then stimulated (a, b) or not (c, d) with 1 mM H₂O₂ for 15 min, and assayed for ROS. RBC (e) and platelets (f) treated with or without Epo were also assayed for GSH. The results, presented as the average (N = 11) of the mean fluorescence index (MFI) ± SD, show a decrease in ROS (P < .05) and an increase in GSH (P < .05) following Epo treatment.