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. 2011 Mar 25;7(3):297–300. doi: 10.7150/ijbs.7.297

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© Ivyspring International Publisher. This is an open-access article distributed under the terms of the Creative Commons License (http://creativecommons.org/licenses/by-nc-nd/3.0/). Reproduction is permitted for personal, noncommercial use, provided that the article is in whole, unmodified, and properly cited.

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Hydrogen treatment protected BALB/c mice from radiation-induced thymic lymphoma. Four weekly sub-lethal doses of 1.75 Gy gamma-ray irradiation were delivered to 4 week old BALB/c mice at a dose rate of 0.58Gy/min as described previously ⁹. These mice were intraperitonealy injected with either H₂-rich saline (H₂ group, H₂ (+)) or normal saline (Control group, H₂ (-)) 5 minutes before each irradiation as described previously ¹³,¹⁴. Panel A; Survival curve analysis of control and H₂ treated mice after split irradiation (N=40). Panel B; tumor incidence at 20 weeks post last irradiation was analyzed by histological study (N=20, 3 repeats). Panel C; Mean latent Period was calculated (N=20, 3 repeats).