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. Author manuscript; available in PMC: 2011 Mar 29.
Published in final edited form as: Kidney Int. 2010 May 26;78(4):363–373. doi: 10.1038/ki.2010.137

Figure 2. Induction of ILK expression in podocytes in response to various injurious stimuli.

Figure 2

Podocytes were treated with 2 µg/ml of adriamycin (a) or with 1 µg/ml of puromycin aminonucleoside (c) for various periods of time as indicated, or with increasing amounts of ADR (b) and PAN (d) for 12 h. Podocytes were also incubated with high glucose (30 mm) for different periods of time as indicated (e). Both normal glucose (5 mm) and mannitol (5 mm normal glucose and 25 mm mannitol) were used as controls and harvested at 9 days. Cell lysates were immunoblotted with antibodies against ILK and housekeeping proteins. ADR, adriamycin; Cont. Man., control mannitol; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; ILK, integrin-linked kinase; PAN, puromycin aminonucleoside.