FIGURE 4.

The effect of Copp on neutrophil HO-1 expression and ${\mathrm{O}}_2^{-}$ production following EtOH intoxication and burn injury. Rats were treated with Copp (25 mg/kg BW) or vehicle at the time of injury. One day after injury, neutrophils were isolated from the blood; HO-1 expression (A) and ${\mathrm{O}}_2^{-}$ production (B) were measured. For HO-1 loading control in various lanes, membranes were erased and reblotted for β-actin. Blots were analyzed using densitometry. The densitometric values were normalized to β-actin and are shown in bar graph as mean ± SEM from four to six animals in each group. For neutrophil ${\mathrm{O}}_2^{-}$ production, we recorded the peak ${\mathrm{O}}_2^{-}$${\mathrm{O}}_2^{-}$ concentration, which was achieved ~20–25 min after neutrophil stimulation with PMA. These peak values were pooled and are shown as mean ± SEM from at least six animals in each group (B). *, p < 0.05 vs other groups in respective panels.