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Design of the LSPR biosensor for serum p53 protein detection in control. A) Ag nanoparticles before chemical modification,λmax = 588.2 nm; B) Ag nanoparticles after modification with 11-MUA/1-OT, λmax = 623.5 nm; C) The chip with 2 μg/mL mouse monoclonal p53 antibody, λmax = 673.84 nm; D) The biochip after incubation with control serum sample, λmax = 676.08 nm. All extinction measurements were collected at room temperature.
