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. 2011 Mar 14;6:495–509. doi: 10.2147/IJN.S16982

Figure 1.

Figure 1

Phase-contrast microscope images of RT4 and T24 cells treated with either cholera toxin B (CTB) or temperature. Images of RT4 A) and T24 B) cells growing in normal conditions without any treatments (control experiments). Note that intercellular membrane nanotubes (ICNs) are not present between RT4 cells, while a few are present between T24 cells (arrow). Following their growth in normal conditions, the cells are moved to room temperature (RT) for 20 minutes, showing no significant change in the morphology of RT4 cells C), while T24 cells become more separated D). In conjunction with 20 minutes at RT, cells are treated with CTB. Note that in RT4 cells E) no ICNs are observed, while many ICNs (arrow) are present between T24 cells F). Finally, the combination of CTB and temperature incubation at 37°C induces rounding of RT4 cells and their detachment G), while T24 cells demonstrate large numbers of ICNs H).