Figure 3.

Cell testing for apoptosis using actin filament and nucleus fluorescence markers. To assess whether exposing cells to an ambient temperature or addition of cholera toxin B (CTB) to RT4 and T24 cells induced apoptosis, cells are immunostained for the integrity labeling of actin filaments with phalloidin-fluorescein isothiocyanate, the integrity of the nucleus with vectashield-4′,6-diamidino-2-phenylindole (top insets), and the integrity of the cells using caspase-3 (bottom insets). Note that neither temperature nor CTB treatments trigger apoptosis in RT4 and T24 cells. However, CTB does cause cell damage in some cells, mostly at 37°C.