Fig. 1. Physical maps of the transgenes used for transient expression analysis. (A) AVR-Pita expression vector. The maize Adh1-6 intron was inserted after the CaMV 35S promoter resulting in a fusion promoter for expression of constructs encoding AVR-Pita₂₂₃, AVR-Pita₁₇₆ and AVR-Pita₁₆₆. (B) GUS expression vector. Plasmid pML63 contained the uidA gene, encoding GUS, under control of the CaMV 35S promoter. The restriction endonucleases used for generating constructs are shown (B, BamHI; E, EcoRI; N, NcoI). Both expression cassettes contain bacterial 3′ nos terminator sequences.