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. 2010 Dec 13;2011:945289. doi: 10.1155/2011/945289

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Copyright © 2011 E. Prus and E. Fibach.

This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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The effects of non-transferrin iron uptake on cytoplasmic and mitochondrial LIP and ROS generation by cultured erythroid precursors. Cultured human erythroid precursors were harvested on day 6 of phase II, incubated with or without 20 μM FAS for 1 hr, and stained with CA, RPA or DCF for measurement of cytoplasmic and mitochondrial LIPs and ROS generation, respectively. The results are expressed as the percent (%) change (mean ± SD, N = 4) in the MFIs following incubation with and without Fe, calculated per the MFI−Fe. (b) The indicated concentrations of FAS were added to day 6 cultures. Hb-containing cells were counted by benzidine staining on day 9. The results are expressed as the (mean ± SD, N = 4).