Fig. 3. Yeast SRP mediates elongation arrest. (A and B) In vitro translation reactions were carried out as in Figure 2 using mRNAs encoding D_HCαF or ppαF (left and right panels, respectively) with or without 100 nM added SRP. SRP was wild type (A) or ΔC29 (B). Samples were stopped at the times indicated. All panels are from the same experiment, and are equivalent exposures processed identically. (C and D) Quantified data from three experiments performed with D_HCαF were averaged and plotted. SRP was wild type (C) or ΔC29 (D). Squares, plus SRP; diamonds, without added SRP.