Fig. 7. Cells expressing ΔC29 have slight translocation defects for Pho8p, but not DPAPB or CPY, at 36°C. Immunoprecipitations were carried out with antibodies against the indicated proteins from extracts of cells pulse-labelled for 6 min (see Materials and methods). Immunoprecipitates were electrophoresed through 10% Proseive gels and visualized by autoradiography. Labelling was at 23°C (srp14-ΔC29, srp14), 30°C (sec63-201) or following transfer from 23 to 36°C for 30 min (sec65-1) or 16 h (srp14-ΔC29, HA-SRP14). Dipeptidyl aminopeptidase B: DPAPB, glycosylated luminal; pDPAPB, cytoplasmic. Alkaline phosphatase: Pho8p, glycosylated luminal; pPho8p, cytoplasmic. Carboxypeptidase Y: gpCPY, glycosylated lumenal; ppCPY, cytoplasmic. In this experiment a band appeared in the sec63-201 sample at the position of pDPAPB. This was not seen in other experiments.