Figure 2.
Effect of inhibitors on phosphatidylserine (PS) exposure in red blood cells (RBCs) from sickle cell patients. RBCs were incubated under deoxygenated conditions (1% O2) for 3 hours (5 mM extracellular [Ca2+]) after which they were labelled with FITC-annexin. Four conditions (all with 0.5% DMSO) are shown: MAPTAM-treated RBCs (loaded with 5 μM MAPTAM prior to deoxygenation), clotrimazole (10 μM), dipyridamole (50 μM), and DIDS (50 μM). Results are presented as percentage PS exposing RBCs relative to control RBCs exposed to 0.5% DMSO only. Histograms represent means ± S.E.M. (n = 3). *P < .01 and # P < .0001 cf DMSO controls.