Table I. Refolding time constants obtained from the recovery of enzymatic activity for the fast track (τ1) and the slow track (τ2) of lysozyme refolding in the absence of crowding agents (–) and in the presence of 200 g/l Ficoll 70 or 100 g/l BSA.
Crowding agent | τ1 (min) | τ2 (min) |
---|---|---|
– | 21.2 ± 1.5 | 187 ± 37 |
Ficoll | 11.6 ± 1.7 | 268 ± 40 |
BSA | 4.3 ± 1.1 | 267 ± 11 |