Table 1.

Vascular Responses to Phenylephrine, Acetylcholine, and Insulin From Aortic Rings in Wild-Type C57BL/6 Mice and in p47^phox−/− Mice^*

Agent	FA-ND	PM2.5-ND	FA-HFD	PM2.5-HFD
C57BL/6 Mice
Phenylephrine
Peak constriction, %	80.1± −4.6	96.0± −2.8†	110.1± −5.5†	131.2± −6.4†‡
EC50, mol/L	1.4×10−7±0.1×10−7	1.3×10−7±0.1×10−7	0.6×10−7±0.2×10−7	0.6×10−7±0.1×10−7
Acetylcholine
Peak relaxation, %	−85.7± −3.5	−82.6± −4.7	−66.5± −4.6	−66.5± −3.8
ED50, mol/L	1.9×10−7±0.1×10−7	4.9×10−7±0.1×10−7†	1.8×10−7±0.2×10−7	2.8×10−7±0.1×10−7‡
Insulin
Peak relaxation, %	−98± −4.6	−105.7± −6.0	−102.1± −7.4	−96.3± −10.5
ED50, mol/L	1.5×10−4± −0.1×10−4	4.5×10−4± −0.1×10−4†	3.4×10−4± −0.2×10−4	7.4×10−4± −0.2×10−4†‡
p47phox−/− Mice
Phenylephrine
Peak constriction, %	88.8±4.5	91.4±5.2	104.9±5.3	100.6±1.9
EC50, mol/L	2.1×10−7±0.1×10−7	3.0×10−7±0.1×10−7	1.8×10−7±0.1×10−7	0.9×10−7±0.1×10−7
Acetylcholine
Peak relaxation, %	−81.1±3.0	−67.0±3.2†	−61.0±2.7	−55.5±2.9
ED50, mol/L	1.9×10−7±0.1×10−7	2.6×10−7±0.1×10−7	1.5×10−7±0.1×10−7	1.5×10−7±0.1×10−7
Insulin
Peak relaxation, %	−102.6±2.6	−108.1±3.4	−105.0±5.6	−100.1±10.9
ED50, mol/L	1.6×10−4± −0.1×10−4	3.9×10−4± −0.1×10−4†	3.1×10−4± −0.1×10−4	7.9×10−4± −0.2×10−4†‡

EC₅₀ indicates the half-maximal dose for constriction; ED₅₀, half-maximal dose for dilation.

^*Data are given as mean±SE.

^†P<0.05 vs FA-ND.

^‡P<0.05 vs FA-HFD.