Isl-1 expression in the developing stomach and intestine. (A) Relative expression of Isl-1 mRNAs in different stages of stomach development from E13.5 to adult measured by real-time PCR (n=4). (B–I) Immunohistochemical staining for Isl-1 in the stomach at E10.5 (B), E11.5 (C), E12.5 (D), E13.5 (E), E14.5 (F–F′), E16.5 (G), E17.5 (H) and P14 (I). Dashed lines outline epithelial compartments. Isl-1+ cells in the epithelium are denoted by “*” in E, G, H, and I. D: Dorsal; V: Ventral; A: Anterior; P: Posterior; mes: mesenchyme and epi: epithelium. (J–L) Immunohistochemical staining for Isl-1 in the intestine at E15.5 (J) and proximal (K) and distal (L) regions of the E17.5 intestine. Isl-1+ cells were denoted by *. (M) Relative expression of Isl-1 mRNAs in different regions of the E17.5 intestine assessed by real-time PCR (n=3). Prox: proximal region; mid1: mid-region 1; mid2: mid-region 2; distal: distal region. Scale bars: 25μm.