Table 1.

Real Time polymerase chain reaction measurements of genes involved with ductus closure and remodeling: ductus obtained from 19d gestation fetuses.

	ΔCT(MDH-gene)						ΔCT(MDH-gene)
Gene	Control		COX Inhibition		P	Gene	Control		COX Inhibition		P
	mean	sd	mean	sd			mean	sd	mean	sd
Ca++ L-channels						Vasodilation
CaLα1c	−2.33	±0.52	−3.09	±0.31	*	COX1	−5.34	±0.37	−5.29	±0.69
CaLβ2	−2.52	±0.34	−3.26	±0.53	*	COX2	−5.53	±0.58	−4.75	±0.89
CaLβ3	−2.71	±0.41	−3.48	±0.52	*	eNOS	−8.86	±0.91	−9.68	±1.43
						EP2	−8.16	±1.00	−7.76	±0.36
K+ channels						EP3	−5.46	±0.27	−5.63	±0.62
BKCa	−9.34	±0.93	−7.99	±1.31		EP4	−0.20	±1.20	−0.71	±0.87
BKCaβ1	−3.70	±0.52	−4.30	±0.36	*	HO1	−3.52	±0.31	−1.48	±0.97	*
Kir6.1	−2.44	±0.72	−3.52	±0.87	*	HO2	−2.51	±0.42	−2.51	±0.28
SUR2	−3.21	±0.43	−3.63	±0.61		PDE1A	−5.22	±0.40	−5.89	±0.19	*
Kv1.2	−4.68	±0.53	−4.99	±0.76		PDE1B	−2.52	±1.03	−2.83	±0.48
Kv1.5	−3.11	±0.36	−3.99	±0.48	*	PDE3A	−2.38	±0.28	−3.41	±0.54	*
Kv2.1	−3.53	±0.83	−3.69	±0.71		PDE3B	−2.80	±0.52	−3.05	±0.13
Kv9.3	−1.47	±0.41	−2.18	±0.53		PDE4D	−6.22	±0.69	−6.51	±0.30
Kvβ1.2	−3.96	±0.63	−4.36	±0.36		PDE5A	−2.75	±0.36	−3.26	±0.55
Kvβ1.3	−1.96	±0.36	−2.29	±0.27
						Remodeling
Contractility						Ang−1	−4.44	±0.54	−4.76	±0.36
Caldesmon	−0.03	±0.21	−0.76	±0.41	*	Ang-2	−5.91	±0.47	−5.82	±0.74
Calponin	−1.58	±0.55	−2.01	±0.49		ATIIR-1	−4.96	±0.72	−5.08	±0.45
Myocardin	−3.17	±0.38	−3.97	±0.31	*	HAS2	−4.69	±0.45	−4.99	±0.19
MYH1	0.41	±0.68	0.05	±0.81		HIF1α	−3.75	±0.87	−3.99	±0.25
MYH2	−1.97	±0.93	−2.34	±0.48		HIF2α	−2.71	±0.66	−2.76	±0.44
RhoA	−0.94	±0.74	−0.80	±0.44		IFNγ	−3.07	±0.54	−3.18	±0.25
RhoB	−2.36	±0.27	−2.87	±0.51		IL6	−3.66	±0.37	−4.06	±0.26
ROCK1	−4.02	±0.81	−4.27	±0.64		PDGF-B	−5.06	±0.45	−5.19	±0.54
NCX1	−4.29	±0.95	−4.92	±0.60		Tenascin-C	−1.60	±0.30	−2.13	±0.36	*
PHLBN	−2.88	±0.68	−2.98	±0.51		TFAP2b	−4.81	±1.34	−5.36	±1.05
SERCA3	−6.58	±0.42	−6.64	±0.23		TGFβ1	−4.17	±0.37	−4.67	±0.29	*
Tropomyosin	−3.00	±0.39	−3.80	±0.54	*	TGFβ3	−2.71	±0.23	−3.43	±0.21	*
						TNFα	−2.38	±1.00	−2.69	±0.29
Endothelin						TRAF1	−5.96	±1.05	−6.31	±0.53
ECE1	−1.18	±0.68	−1.86	±0.26		VEGF	−3.57	±0.50	−2.78	±0.42	*
ET1	−4.43	±0.95	−4.55	±0.62
ETAr	−5.99	±1.52	−6.27	±1.67
ETBr	−2.66	±0.79	−2.77	±0.37

ΔCT(MDH-gene) = difference in cycle threshold (CT) between the expression of the housekeeping gene Malate dehydrogenase (MDH) and the gene of interest. Each unit of ΔCT(MDH-gene) represents a 2-fold increase in a gene’s mRNA. The more negative the ΔCT(MDH-gene), the fewer the number of starting copies of a gene (mRNA). Number of separate litters used: Control (day 19) = 9, COX-inhibition (days 15–19) = 7. From each litter of mice we obtained and pooled between 10–12 ductus.

^*p<0.05.

MYH, myosin heavy chain; ROCK1, Rho kinase 1; NCX1, Na⁺/Ca⁺⁺ exchanger; PHLBN, phospholamban; SERCA, sarcoplasmic reticulum Ca⁺⁺-ATPase; ECE, endothelin converting enzyme; ET, endothelin; ETAr, endothelin receptor A; eNOS, endothelial nitric oxide synthase; EP, prostaglandin E receptor; HO, heme oxygenase; PDE, phosphodiesterase; ang, angiopoietin; ATIIR-1, angiotensin II receptor; HAS, hyaluronic acid synthase; HIF, hypoxia inducible factor; IFN, interferon; IL, interleukin; PDGF, platelet derived growth factor.