Table 2.

Real Time PCR measurements in sheep ductus.

	ΔCT(MDH-gene)						ΔCT(MDH-gene)
Gene	Control		PGE2 incubation		P	Gene	Control		PGE2 incubation		P
	mean	sd	mean	sd			mean	sd	mean	sd
Genes affected by chronic COX inhibition in utero						Genes un-affected by chronic COX inhibition in utero
CaLα1c	−0.24	±0.49	0.59	±0.54	*	BKCa	−7.13	±0.98	−7.27	±0.55
CaLβ2	−2.31	±1.01	−1.33	±0.83	*	COX2	0.77	±0.96	1.06	±1.36
CaLβ3	−1.28	±0.38	−1.12	±0.31		EP2	−1.86	±0.43	−1.96	±0.85
Caldesmon	1.12	±0.78	1.11	±0.63		EP3	−5.44	±1.03	−4.99	±0.98
BKCaβ1	−4.40	±0.26	−4.06	±0.44		ET1	−4.52	±1.33	−4.74	±1.22
HO1	−2.08	±0.38	−1.59	±0.47	*	PDGF−B	−3.95	±0.83	−3.91	±0.71
Kir6.1	−5.21	±1.00	−4.31	±0.92	*	RhoA	1.87	±0.41	2.07	±0.39
Kv1.5	−1.99	±1.40	−0.96	±0.87	*	RhoB	2.42	±0.61	2.80	±0.78
Myocardin	−3.40	±0.47	−3.29	±0.77		ROCK1	−2.82	±0.75	−2.49	±0.59
PDE1A	−5.79	±1.02	−4.72	±0.95	*	SUR2	−1.04	±0.41	−0.78	±0.55
PDE3A	−2.58	±0.66	−1.62	±0.96	*
Tenascin-C	1.00	±0.81	0.64	±1.18
TGFβ1	−0.61	±0.34	−0.35	±0.31
TGFβ3	−4.09	±0.76	−3.76	±0.64
Tropomyosin	−0.25	±1.01	−0.50	±0.58
VEGF	0.31	±0.72	1.61	±0.86	*

Rings from the same sheep ductus were incubated with or without PGE2 (10⁻⁸ M) for 20 hours. We examined the expression of genes that were altered by chronic COX inhibition in utero in Table 1; we also examined the expression of several of the genes that were not affected by chronic COX inhibition (see Table 1). Number of separate animals used = 7.

^*p<0.05.