Fig. 1. Electron micrograph of osmotically shocked M94 cells. Cells were negatively stained with 2% phosphotungstic acid pH 7.0 and observed under TEM. The open arrowheads indicate the type III secretion complexes on the bacterial envelope and the closed arrowhead denotes the bleb-like moiety associated with the tip of the complex, reminiscent of the secreted proteins through the type III secretion complexes. Scale bar, 100 nm.