Fig. 5. Analysis of the mxiH mutant and its complemented mutant for the type III secretion complexes. (A) Genomic organization of the mxi operon and construction of the mxiH::aphA-3 mutant. (B) Pattern on the 20% SDS–polyacrylamide gel (upper part) and immunoblot (lower part) with antibodies specific for MxiD, MxiG and MxiJ of partially purified type III secretion complexes of M94, the mxiH mutant (mxiH^–), the mxiH mutant harboring pKT001 (mxiH^–/mxiH), del-17 and YSH6200. The gel was stained with CBB. (C) Electron micrographs of the purified type III secretion complexes from the mxiH mutant. Scale bar, 20 nm.