Fig. 1. srw1p is not required for cdc13p degradation at mitotic exit. (A) Septation index and (B) flow cytometric analysis of G₂ block and release experiments. cdc25-22 and cdc25-22 srw1Δ strains were grown to exponential phase at 25°C (cyc), blocked for 4 h at 36°C, released at 25°C and incubated for the time indicated. (C) Western blots of the experiments described above using anti-cdc13p, anti-phosphotyrosine cdc2p, anti-rum1p and anti-cdc2p antibodies. For anti-rum1p antibody blotting, the same blots were used after the anti-phosphotyrosine cdc2p antibody.