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. Author manuscript; available in PMC: 2011 Mar 29.
Published in final edited form as: J Biol Chem. 2006 Apr 19;281(24):16401–16409. doi: 10.1074/jbc.M513267200

FIGURE 3. Expression of E. coli UbiF rescues the E194KCoq7 point mutant more effectively than the coq7 null mutant.

FIGURE 3

Each strain was grown overnight in selective media and serial dilutions were spotted on SDC, YPG, YPE, and YPEG plate media, at a final optical density 600 nm (A600) of 0.2, 0.02, 0.002, or 0.0002. Growth is depicted after 6 days at 30 °C. E194K, Δ, and WtCoq7, represent E194KCoq7, the coq7 null mutant (JM43Δcoq7-1) or the wild-type integrant, respectively. JM43 designates the parental wild-type strain. Yeast strains were transformed with pQMF, a low copy plasmid expressing E. coli UbiF; pCHF, a multicopy plasmid expressing E. coli UbiF; or pNMQ71, a low copy plasmid containing the yeast COQ7 gene.