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. Author manuscript; available in PMC: 2011 Mar 29.
Published in final edited form as: J Immunol. 2010 Jan 18;184(4):2014–2025. doi: 10.4049/jimmunol.0900183

FIGURE 1.

FIGURE 1

In vivo and ex vivo screening assays confirmed enhanced anti-influenza immune responses in SDR-MEM mice. A, SDR-MEM mice developed a significantly greater DTH response over time versus nonstressed memory controls (MEM) following s.c. footpad injection with influenza A/PR/8/34 Ag. Each animal served as its own control, with one saline-injected and one Ag-injected footpad measured at 24-h intervals. Values were analyzed by subtracting the saline-injected footpad thickness from the Ag-injected footpad thickness at each time point (RM-ANOVA; SDR > MEM; p < 0.001; n = 20 per group). Values shown represent group mean ± SEM. This dataset is representative of four separate experiments. Mixed cell preparations from individual spleen (B) or lung (C) tissues taken from MEM or SDR-MEM mice at 6 wk post-A/PR/8/34 infection were cocultured with UV-PR8 for 6 h and then the anti-influenza CD4+ T cell response was assessed via flow cytometry. Organs were analyzed individually with n = 5–7 per group. *p < 0.05.