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. Author manuscript; available in PMC: 2011 Mar 29.
Published in final edited form as: Methods Mol Biol. 2010;615:227–246. doi: 10.1007/978-1-60761-535-4_18

Figure 2.

Figure 2

Biochemical validation of the fractionation protocol. To demonstrate the enrichment of proteins in each fraction, equal amounts of protein from each fraction were separated by SDS-PAGE and probed with antibodies for presynaptically (Syntaxin 1) and postsynaptically (PSD95) enriched proteins.