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. Author manuscript; available in PMC: 2011 Mar 29.
Published in final edited form as: J Allergy Clin Immunol. 2010 Dec 17;127(2):462–469. doi: 10.1016/j.jaci.2010.10.029

FIG 6.

FIG 6

Adenosine receptor antagonists do not induce relaxation and partially reverse the effect of R837-mediated relaxation of isolated guinea pig trachea in vitro. A, Contraction of tracheal segments was induced by methacholine, and the effect of cumulative increasing doses of R837 (circles), the adenosine A1 receptor antagonist DPCPX (diamonds), or the adenosine A2A receptor antagonist SCH58261 (triangles) was measured (n = 3; maximum, 1.29 ± 0.10 g; effect of dose of R837, P ≤ .001). B, Contraction of tracheal segments was induced by methacholine and incubated in the absence or presence of SCH58261, DPCPX, or SCH58261 and DPCPX together, and the effect of R837 was measured (n = 3; maximum, 1.3 ± 0.16 g; R837 vs 2 µmol/L DPCPX, P ≤ .05). C, Tracheal segments were preincubated with vehicle (open bars) or IRS661 (solid bars). Contraction of tracheal segments was induced by methacholine, and the effect of 5′-(N-ethylcarboxamido)adenosine (NECA) was measured (n = 2; maximum, 1.78 ± 0.30 g).