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. Author manuscript; available in PMC: 2012 Mar 1.
Published in final edited form as: Nat Protoc. 2011 Feb 17;6(3):296–313. doi: 10.1038/nprot.2010.184

Figure 4.

Figure 4

Demonstration of morphological changes in OP9 associated with a loss of hematopoiesis-inductive properties. (a) Spontaneous adipogenesis in overgrown OP9 cells. (b) After 8 days of hPSC/adipogenic OP9 coculture, the majority of hPSC colonies retain undifferentiated morphology. (c) Heterogeneous overgrown OP9 cultures with spindle cells forming long cords. (d) After 8 days of hPSC/heterogeneous OP9 coculture, the majority of hPSC colonies retain undifferentiated morphology. Scale bars=300µm.

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