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| Composition | Volume | Final concentration |
|---|---|---|
| Differentiation (hPSC/OP9 coculture) medium | - | |
| GM-CSF (100 µg/ml) | 1/500 dilution | 200 ng/ml |
Volume of the medium will vary depending on input cell number. In this protocol, we use 1 ml of medium for ~1×106 cocultured cells. The complete medium is prepared freshly and GM-CSF is added right before use.
