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| Composition | Volume | Final concentration |
|---|---|---|
| IMDM with 20% FBS medium | - | |
| IL-3 (10 µg/ml) | 1/1000 dilution | 10 ng/ml |
| IL-5 (10 µg/ml) | 1/2000 dilution | 5ng/ml |
Volume of the medium will vary depending on input cell number. In this protocol, we use 1ml of medium for 2×104 cells. The complete medium is prepared freshly and all cytokines and supplements are added right before use.
