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| Composition | Volume | Final concentration |
|---|---|---|
| IMDM with 10% FBS medium | - | |
| M-CSF (10 µg/ml) | 1/500 dilution | 20 ng/ml |
| IL-1β (10 µg/ml) | 1/1000 dilution | 10 ng/ml |
Volume of the medium will vary depending on input cell number. In this protocol, we use 1ml of medium for 105 cells. The complete medium is prepared freshly and all cytokines and supplements are added right before use.
