Table 2.
Antibodies used to analyze differentiation of hematopoietic progenitors and myeloid lineages from human pluripotent stem cells
| Name | Fluorov chrome | Clone | Company | Cat. no. |
|---|---|---|---|---|
| Anti-human CD1a | PE | VIT6b | CALTAG-Invitrogen | MHCD1a04 |
| Anti-human CD2 | FITC | RPA-2.10 | BD Biosciences | 555326 |
| Anti-human CD3 | FITC | SK7 | BD Biosciences | 349201 |
| Anti-human CD7 | PE | M-T701 | BD Biosciences | 555361 |
| Anti-human CD10 | PE | HI10a | BD Biosciences | 555375 |
| Anti-human CD11b | FITC | VIM12 | CALTAG-Invitrogen | CD11b01 |
| Anti-human CD13 | PE | TüK1 | CALTAG-Invitrogen | MHCD1304 |
| Anti-human CD14 | FITC | M5E2 | BD Biosciences | 555397 |
| Anti-human CD15 | FITC | VIMC6 | Miltenyi Biotec | 130-081-101 |
| Anti-human CD16 | PE | 3G8 | CALTAG-Invitrogen | MHCD1604 |
| Anti-human CD19 | PE | HIB19 | BD Biosciences | 555413 |
| Anti-human CD34 | APC | 581 | BD Biosciences | 555824 |
| Anti-human CD41a | PE | HIP8 | BD Biosciences | 555467 |
| Anti-human CD43 | FITC | 1G10 | BD Biosciences | 555475 |
| Anti-human CD45 | APC | HI30 | BD Biosciences | 555485 |
| Anti-human CD64 | FITC | 10.1 | CALTAG-Invitrogen | CD6401 |
| Anti-human CD66b | FITC | G10F5 | BD Biosciences | 555724 |
| Anti-human CD90 (Thy-1) | APC | 5E10 | BD Sciences | 559869 |
| Anti-human CD115 | PE | 61708 | R&D Systems | FAB329P |
| Anti-human CD117 | APC | YB5.B8 | BD Biosciences | 550412 |
| Anti-human CD123 | FITC | AC145 | Miltenyi Biotec | 130-090-897 |
| Anti-human CD163 | PE | 215927 | R&D Systems | FAB1607P |
| Anti-human CD235a | PE | GA-R2(HIR2) | BD Biosciences | 555570 |
| DC-SIGN | FITC | DCN46 | BD Biosciences | 551264 |
| HLA-DR | PE | Tü36 | CALTAG-Invitrogen | MHLDR04 |
| Lactoferrin* | PE | 3C5 | CALTAG-Invitrogen | GIC206 |
| Langerin* | PE | 343828 | R&D Systems | FAB2088P |
| Major basic protein (MBP)* | None | AHE-2 | BD Biosciences | 550843 |
| Myeloperoxidase (MPO)* | FITC | H-43-5 | CALTAG-Invirogen | GIC205 |
| Anti-human TRA-1-85 | APC | TRA-1-85 | R&D Systems | FAB3195A |
*
represent intracellular staining.
BD Biosciences (San Jose, CA, USA; http://www.bdbiosciences.com), CALTAG-Invitrogen (Carlsbad, CA, USA; http://www.invitrogen.com), R&D Systems (Minneapolis, MN, USA; http://www.rndsystems.com), Miltenyi Biotec(Begisch Gladbach, Germany; http://www.miltenyibiotec.com), Biolegend(San Diego, CA, USA; http://www.biolegend.com).