. Author manuscript; available in PMC: 2012 Mar 1.

Published in final edited form as: Nat Protoc. 2011 Feb 17;6(3):296–313. doi: 10.1038/nprot.2010.184

Table 3.

Reconstitution of cytokines

Cytokines	Product state	Reconstitution	Working dilution	Storage
Neupogen as a human G-CSF	Concentrated aqueous solution	-	100 µg/ml in 0.1% BSA/PBS solution	Aliquot 100 µl into autoclaved 0.5 ml tubes and store at −80°C until needed for use.
Leukine as a human GM-CSF	Concentrated aqueous solution	-	100 µg/ml in 0.1% BSA/PBS solution	Aliquot 100 µl into autoclaved 0.5 ml tubes and store at −80°C until needed for use.
Human FGF-basic	Lyophilized pellet	1 mg/ml with 5mM Tris (pH7.6)	100 µg/ml in 0.1% BSA/PBS solution	Aliquot 100 µl into autoclaved 0.5 ml tubes and store at −80°C until needed for use.
Human Flt3-Ligand	Lyophilized pellet	1 mg/ml with distilled water	100 µg/ml in 0.1% BSA/PBS solution	Aliquot 100 µl into autoclaved 0.5 ml tubes and store at −80°C until needed for use.
Human IL-1β	Lyophilized pellet	100 µg /ml with distilled water	10 µg/ml in 0.1% BSA/PBS solution	Aliquot 100 µl into autoclaved 0.5 ml tubes and store at −80°C until needed for use.
Human IL-3	Lyophilized pellet	100 µg /ml with distilled water	10 µg/ml in 0.1% BSA/PBS solution	Aliquot 100 µl into autoclaved 0.5 ml tubes and store at −80°C until needed for use.
Human IL-4	Lyophilized pellet	1mg/ml with distilled water	100 µg/ml in 0.1% BSA/PBS solution	Aliquot 100 µl into autoclaved 0.5 ml tubes and store at −80°C until needed for use.
Human IL-5	Lyophilized pellet	100 µg /ml with distilled water	10 µg/ml in 0.1% BSA/PBS solution	Aliquot 100 µl into autoclaved 0.5 ml tubes and store at −80°C until needed for use.
Human M-CSF	Lyophilized pellet	100 µg /ml with distilled water	10 µg/ml in 0.1% BSA/PBS solution	Aliquot 100 µl into autoclaved 0.5 ml tubes and store at −80°C until needed for use.
Human sRANKL	Lyophilized pellet	100 µg /ml with distilled water	10 µg/ml in 0.1% BSA/PBS solution	Aliquot 100 µl into autoclaved 0.5 ml tubes and store at −80°C until needed for use.
Human TGF-β₁	Lyophilized pellet	50 µg/ml with 10 mM citric acid solution (pH 3.0)	5 µg/ml in 0.1% BSA/PBS solution	Aliquot 100 µl into autoclaved 0.5 ml tubes and store at −80°C until needed for use.
Human TNF-α	Lyophilized pellet	1mg/ml with distilled water	50 µg/ml in 0.1% BSA/PBS solution	Aliquot 100 µl into autoclaved 0.5 ml tubes and store at −80°C until needed for use.

Vials of cytokine products in lyophilized pellet state should be centrifuged at maximum speed for 1 minutes with microcentrifuge to precipitate pellet prior to opening vials. Distilled water and all buffers should be sterilized by filtration with 0.22 µm membrane filter.