| Composition | Volume | Final concentration |
|---|---|---|
| DMEM | 445 ml | |
| FBS | 50 ml | 10% |
| 100× NEAA solution (10 mM) | 5 ml | 100 µM |
Sterilize the medium by filtration using a 0.22 µm membrane filter and store for up to 3 weeks at 2–8°C
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