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| Composition | Volume | Final concentration |
|---|---|---|
| α-MEM | 450 ml | 90% |
| FBS | 50 ml | 10% |
| 1000× MTG solution (100 mM) | 500 µl | 100 µM |
Sterilize the medium by filtration using a 0.22 µm membrane filter and store for up to 2 weeks at 2–8°C. Optional: Ascorbic acid solution (50 mg/ml) can be added to achieve 50 µg/ml final concentration.
