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. Author manuscript; available in PMC: 2011 Mar 29.
Published in final edited form as: J Immunol. 2009 Dec 16;184(2):615–623. doi: 10.4049/jimmunol.0902408

FIGURE 1.

FIGURE 1

IL-4Rα expression is oppositely influenced by cytokine-mediated and antigenic stimulation. A, CD4+ CD44lo cells sorted from naive DO11.10 animals (CD90.1−, specific for the OVA peptide 323–339 [pOVA]) were mixed 1:1 with equivalent cells from HNT mice (CD90.1+, specific for the hemagglutinin peptide 126–138 [pHA]) and cultured in the presence of irradiated APCs. Medium was supplemented with recombinant IL-4 or peptide Ag as indicated. Cells were recovered 24 h later and analyzed by flow cytometry for expression of IL-4Rα and CD25. B, Identical cocultures were established, and pHA-stimulated cells were analyzed for dual expression of IL-4Rα and CD25, gating on each transgenic population as shown. In both cases, data are representative of five independent experiments.