Table 5.

Recombinant DCK and CMPK allozyme substrate kinetics.

DCK Allozyme	Apparent Km (µM)
WT	14.6 ± 3.5
Val24	13.1 ± 3.2
Gly119	14.5 ± 2.5
Ser122	14.1 ± 3.3
Val24/Gly119/Ser122	14.8 ± 2.5
Gly119/Ser122	9.5 ± 2.5
Val24/Gly119	10.7 ± 1.1
Val24/Ser122	6.3 ± 0.8*
CMPK Allozyme	Apparent Km (µM)
WT	246 ± 61
His48	208 ± 45
Lys75	265 ± 67
Ser83	236 ± 39

Gemcitabine substrate kinetics for WT and variant DCK allozymes and gemcitabine monophosphate substrate kinetics for CMPK were measured in the presence of 1 mM ATP. Values represent mean ± SEM (n ≥ 6).

^*Indicates a statistically significant difference from the WT allozyme (P < 0.025 by Student’s t-test).