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. Author manuscript; available in PMC: 2011 Mar 29.
Published in final edited form as: Cell Mol Bioeng. 2010 Jun 1;3(2):151–162. doi: 10.1007/s12195-010-0119-x

FIGURE 4.

FIGURE 4

Cells were plated onto fibronectin-coated glass bottom dishes containing control, cholesterol-depletion or cholesterol-enrichment solutions. Total spreading area was observed for untreated control cells (N = 40), cholesterol depleted (N = 60) and cholesterol enriched cells (N = 53) after 1 h of spreading using IRM. ImageJ was used to trace cell boundaries and quantify total spreading area. Error bars illustrate standard error with statistical significance reported using Student’s t test (p <0.01). Depleted cells are statistically less spread compared to control cells, while enriched cells are not statistically different.