Fig. 3. Interaction of lamin B₃ with the C-terminus of Nup153 in blot overlay assays. Nup153 was expressed as three fragments (residues 2–609, 610–895 and 896–1475, respectively) in E.coli and partially purified. The fragments were resolved on SDS–PAGE and either stained with Coomassie Blue (A) or transferred to nitrocellulose and overlayed with [³⁵S]methionine-labelled lamin B₃ (B), [³⁵S]methionine-labelled lamin A (C) or [³⁵S]methionine-labelled emerin (D). After extensive washing, the nitrocellulose filters were dried and autoradiographed. Arrows show the positions of the full-length recombinant peptides. Molecular weight markers are 97.5, 66.3, 45, 31, 21.5 and 14.5 kDa, respectively.