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. 2011 Mar 18;8:127. doi: 10.1186/1743-422X-8-127

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Copyright ©2011 Nogueira et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Expression of the T. cruzi TEWETGQI epitope by recombinant YF17D/E200/Tc and YF17D/NS2B3/Tc viruses. Indirect immunofluorescence assay of Vero cells infected with recombinant viruses, YF 17DD vaccine virus or not infected (Earle's Medium 199) stained with a mouse polyclonal hyperimmune serum to YF17D (HIYF) or polyclonal antibody directed to the TEWETGQI epitope. Second antibodies were Alexa Fluor 488 goat anti-mouse IgG and Alexa Fluor 546 goat anti-mouse IgG for HIYF (green color) or anti-TEWETGQI (red color) preparations, respectively.