Figure 4.

SERPINE1 mRNA level in fibroblasts under hypoxia (Figure 6). Transfection of miRNA-449a/b inhibitors additionally increased the hypoxia-induced SERPINE1 mRNA expression. The mean, minimum and maximum of calculations relative to reference gene POLR2A compared with non-transfected cells (= control) or cells transfected with negative control siRNA only (= negative control) cultured under hypoxia in 3 independent experiments are depicted. The miRNA-449 mimics showed virtually no down-regulation of SERPINE1 mRNA level.