Fig. 3. Degradation of cdc13 and cig1 in mitosis does not require ste9 or rum1. Wild-type, ste9Δ, rum1Δ and ste9Δ rum1Δ cells were synchronized in G₂ using the cdc25-22 mutant. After 4 h at 36°C, the cultures were release to 25°C and samples were taken to measure cig1, cdc13 and α-tubulin levels.