Fig. 1.
Expression of COX1 and COX2 in rhesus monkey neocortex and specificity of COX1 and COX2 antibodies. Rows demonstrate immunohistochemistry (IHC) against COX1 (A–C) and COX2 (D–F). Columns demonstrate preincubation of antibodies with no peptide (A, D), heterologous peptide (B, E) and homologous peptide (C, F). As shown in representative low-and medium-power images of IHC on adjacent sections COX1 (A with inset) and COX2 (D with inset) are detected in neurons in the occipital cortex of a control rhesus monkey. The antibodies recognize specifically their cognate peptides as their immunostaining are not preabsorbed with heterologous peptides (B, E), but with excess homologous peptides (C, F). Scale bars = (A–F) 200 µm; (insets in A–F) 100 µm.