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. 2011 Mar 29;5(3):e1000. doi: 10.1371/journal.pntd.0001000

Figure 1. Elimination of Trypanosoma cruzi infection early in Gallus gallus embryonic development.

Figure 1

A) Top panel shows 330 bp bands formed by PCR amplified minicircles kDNA templates harvested at several stages of the chicken embryonic development, after hybridization with a specific probe; Bottom panel shows bands formed by PCR amplified from same embryos after separation in 1% agarose gel and hybridization with a specific nDNA probe; the 188 bp nDNA band was diagnostic of the parasite persistence in the host tissue. B) Sensitivity of the PCR with nDNA primers Tcz1/2. Lanes 1 and 2, control DNA from kDNA negative and from kDNA-mutated chickens; Lanes 3 to 7, mix of 200 ng of control chicken DNA with increasing amounts of T. cruzi DNA, respectively: 1 fg, 10 fg, 1 pg, and 100 pg, and 1 ng. The hybridization with the radiolabeled 188-bp probe improved the technique sensitivity (10 fg), which reached 24-fold below the diploid T. cruzi total DNA.