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. 2011 Mar 29;5(3):e1000. doi: 10.1371/journal.pntd.0001000

Figure 3. Retention of the kDNA minicircle from Trypanosoma cruzi in the Gallus gallus genome.

Figure 3

A) PCR amplification of kDNA from 12 adult chickens hatched from T. cruzi-infected eggs with primer set s35/s36 and hybridization with NsiI-digested, whole kDNA labeled as probe. The lower panel shows the absence of nDNA by the Tcz1/2 primer set, hybridized with a 188 bp probe. B) G. gallus somatic cell's DNA templates from F3 progeny, which were separated in 1% agarose gels, revealed the minicircle 330-bp band in the absence of nDNA by using specific primer sets and hybridization with the 188 bp probe. C) G. gallus germ line DNA templates from parental 1 and 2, and from mated progeny in the F1 (8 and 9) and F2 (19 and 20) amplified with primer sets s35/s36 or Tcz1/2 and probed as described.