Table 1. Probes used in the tpTAIL-PCR amplifications.

Primer	Target DNA	Sequence	Tm*
S 34	T. cruzi kDNA	5′ ACA CCA ACC CCA ATC GAA CC 3′	57,9
S 67	T. cruzi kDNA	5′ GGT TTT GGG AGG GG(G/C) (G/C)(T/G)T C 3′	60,1
S 35	T. cruzi kDNA	5′ ATA ATG TAC GGG (T/G)GA GAT GC 3′	59,4
S 36	T. cruzi kDNA	5′ GGT TCG ATT GGG GTT GGT G 3′	57,9
Gg1	G. gallus	5′ AGC TGA TCC TAA AGG CAG AGC 3′	60.1
Gg2	G. gallus	5′ CTG AGC CTC TGC TTT GAA A 3′	56.8
Gg3	G. gallus	5′ TTT CAA AGC AGA GGC TCG G 3′	60.1
Gg4	G. gallus	3′ GCT CTG CCT TTA GGA TCA GCT 5′	64.2
Gg5	G. gallus	3′ AGC AAC TCA GCG TCC ACC TT 5′	62.3
Gg6	G.gallus	3′ CTG TTA GCA TGA GGC TTC ACA A 5′	60.4
XeCRs-1a	G. gallus	5′ ATW TCW GTS TTT GCA GAT GAC ACA 3′	60.4
XeCRs-2	G. gallus	5′ CTT WGT TGC CCT YCT CTG KAC YCT CTC YA 3′	66.6
XeCRs-3	G. gallus	5′ TGT GTC ATC TGC AAA SAC WGA WAT 3′	65.3
XeCRs-4	G. gallus	5′TRG AGA GRG TMC AGA GRA GGG CAA CWA TG	3′ 67.9

*Tm  =  average annealing temperature °C.

^aXeCrs primer sets were a gift from Professor Dusan Kordis to Dr. Jiri Hejnar, Czech Academy of Sciences, Praha.