Fig. 12. Vaccinia infection dramatically reduces levels of centrosomal components. Immunofluorescent γ-tubulin labelling of centrosomes in uninfected control cells (A and B) and in cells 2 h post-infection with vaccinia (C and D) at similar stages of the cell cycle. All images from the same experiment were collected with identical camera settings, to allow comparison of fluorescence intensity. Inserts show the corresponding images with a 5-fold increase in brightness and 3-fold decrease in midtones, to facilitate visualization of the weak γ-tubulin centrosomal labelling in infected cells. The effects of a 2 h vaccinia infection on centrosomal levels of pericentrin (E and F), C-Nap 1 (G and H), Nek 2 (I and J) and centrin (K and L) are also shown. Arrowheads indicate the position of weakly labelled centrosomes in infected cells. Scale bar = 10 µm.