Fig. 5. IEV formation is microtubule and Golgi dependent. IEV particles, which are identified by co-labelling with antibodies against A27L and the A36R IEV membrane protein (A–C), are not formed in the presence of nocodazole (D–F) or brefeldin A (G–I). Actin tails normally induced by IEV (J–L) are also absent in nocodazole- (M–O) or brefeldin A- (P–R) treated cells. Scale bar = 10 µm.