Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Activity assay of PhDph2 single mutants monitored by autoradiography and HPLC. a. 14C- labeling of PhEF2 by PhDp2 wild type and three single mutants. The reactions contain PhEF2, PhDph2 (WT or mutants), SAM, and dithionite. Left panel shows the Coomassie Blue-stained gel and right panel shows the autoradiography. b. Reaction product methylthioadenosine (MTA) formation was detected by HPLC. SAM was eluted at 2 min and MTA was eluted at 21.5 min.
